8GIS

Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.5mM Mn2+

8GIS の概要

• エントリーDOI: 10.2210/pdb8gis/pdb
• 分子名称: Cyclic GMP-AMP synthase, Palindromic DNA18, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: transferase-dna complex, cgas, atp and divalent metal ion, immune system, transferase/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 108703.85

構造登録者

Wu, S.,Sohn, J. (登録日: 2023-03-14, 公開日: 2024-04-17, 最終更新日: 2024-05-29)

主引用文献

Wu, S.,Gabelli, S.B.,Sohn, J.
The structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS.
Nat Commun, 15:4012-4012, 2024

PubMed Abstract: cGAS activates innate immune responses against cytosolic double-stranded DNA. Here, by determining crystal structures of cGAS at various reaction stages, we report a unifying catalytic mechanism. apo-cGAS assumes an array of inactive conformations and binds NTPs nonproductively. Dimerization-coupled double-stranded DNA-binding then affixes the active site into a rigid lock for productive metal•substrate binding. A web-like network of protein•NTP, intra-NTP, and inter-NTP interactions ensures the stepwise synthesis of 2'-5'/3'-5'-linked cGAMP while discriminating against noncognate NTPs and off-pathway intermediates. One divalent metal is sufficient for productive substrate binding, and capturing the second divalent metal is tightly coupled to nucleotide and linkage specificities, a process which manganese is preferred over magnesium by 100-fold. Additionally, we elucidate how mouse cGAS achieves more stringent NTP and linkage specificities than human cGAS. Together, our results reveal that an adaptable, yet precise lock-and-key-like mechanism underpins cGAS catalysis.

PubMed: 38740774
DOI: 10.1038/s41467-024-48365-3

実験手法

X-RAY DIFFRACTION (2.46 Å)