8GF6

Apo-apo MCR assembly intermediate

8GF6 の概要

• エントリーDOI: 10.2210/pdb8gf6/pdb
• 関連するPDBエントリー: 8GF5
• EMDBエントリー: 29978 29979
• 分子名称: Methyl-coenzyme M reductase subunit alpha, Methyl-coenzyme M reductase subunit beta, Methyl-coenzyme M reductase subunit gamma, ... (4 entities in total)
• 機能のキーワード: methanogenesis, mcr complex, transferase
• 由来する生物種: Methanosarcina acetivorans C2A; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 291811.41

構造登録者

Joiner, A.M.N.,Chadwick, G.L.,Nayak, D.D. (登録日: 2023-03-07, 公開日: 2023-06-28)

主引用文献

Chadwick, G.L.,Joiner, A.M.N.,Ramesh, S.,Mitchell, D.A.,Nayak, D.D.
McrD binds asymmetrically to methyl-coenzyme M reductase improving active-site accessibility during assembly.
Proc.Natl.Acad.Sci.USA, 120:e2302815120-e2302815120, 2023

PubMed Abstract: Methyl-coenzyme M reductase (MCR) catalyzes the formation of methane, and its activity accounts for nearly all biologically produced methane released into the atmosphere. The assembly of MCR is an intricate process involving the installation of a complex set of posttranslational modifications and the unique Ni-containing tetrapyrrole called coenzyme F. Despite decades of research, details of MCR assembly remain largely unresolved. Here, we report the structural characterization of MCR in two intermediate states of assembly. These intermediate states lack one or both F cofactors and form complexes with the previously uncharacterized McrD protein. McrD is found to bind asymmetrically to MCR, displacing large regions of the alpha subunit and increasing active-site accessibility for the installation of F-shedding light on the assembly of MCR and the role of McrD therein. This work offers crucial information for the expression of MCR in a heterologous host and provides targets for the design of MCR inhibitors.

PubMed: 37307484
DOI: 10.1073/pnas.2302815120

実験手法

ELECTRON MICROSCOPY (3.1 Å)