8GEX

Crystal structure of the ferric enterobactin transporter (XusB) from Bacteroides thetaiotaomicron

8GEX の概要

• エントリーDOI: 10.2210/pdb8gex/pdb
• 分子名称: DUF4374 domain-containing protein (2 entities in total)
• 機能のキーワード: transport protein
• 由来する生物種: Bacteroides thetaiotaomicron
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 278845.08

構造登録者

Perera, Y.R.,Chazin, W.J. (登録日: 2023-03-07, 公開日: 2023-08-02, 最終更新日: 2024-08-14)

主引用文献

Spiga, L.,Fansler, R.T.,Perera, Y.R.,Shealy, N.G.,Munneke, M.J.,David, H.E.,Torres, T.P.,Lemoff, A.,Ran, X.,Richardson, K.L.,Pudlo, N.,Martens, E.C.,Folta-Stogniew, E.,Yang, Z.J.,Skaar, E.P.,Byndloss, M.X.,Chazin, W.J.,Zhu, W.
Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.
Cell Host Microbe, 31:1639-1654.e10, 2023

PubMed Abstract: During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients, such as iron. Pathogens scavenge iron using siderophores, including enterobactin; however, this strategy is counteracted by host protein lipocalin-2, which sequesters iron-laden enterobactin. Although this iron competition occurs in the presence of gut bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron and sustains its resilience in the inflamed gut by utilizing siderophores produced by other bacteria, including Salmonella, via a secreted siderophore-binding lipoprotein XusB. Notably, XusB-bound enterobactin is less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella, allowing the pathogen to evade nutritional immunity. Because the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the host-pathogen interactions and nutritional immunity.

PubMed: 37776864
DOI: 10.1016/j.chom.2023.08.018

実験手法

X-RAY DIFFRACTION (2.55 Å)