8GBS

Integrative model of the native Ana GV shell

これはPDB形式変換不可エントリーです。

8GBS の概要

• エントリーDOI: 10.2210/pdb8gbs/pdb
• EMDBエントリー: 29921 29922 29923 29924 29925
• 分子名称: Gas vesicle structural protein, Gas vesicle protein C (2 entities in total)
• 機能のキーワード: gas vesicles, flotation, cyanobacteria, cytosolic protein
• 由来する生物種: Dolichospermum flos-aquae (Anabaena flos-aquae); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 32965.36

構造登録者

Dutka, P.,Metskas, L.A.,Hurt, R.C.,Salahshoor, H.,Wang, T.U.,Malounda, D.,Lu, G.,Chou, T.F.,Shapiro, M.G.,Jensen, J.J. (登録日: 2023-02-28, 公開日: 2023-04-19, 最終更新日: 2024-06-19)

主引用文献

Dutka, P.,Metskas, L.A.,Hurt, R.C.,Salahshoor, H.,Wang, T.Y.,Malounda, D.,Lu, G.J.,Chou, T.F.,Shapiro, M.G.,Jensen, G.J.
Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET.
Structure, 31:518-528.e6, 2023

PubMed Abstract: Gas vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryoelectron tomography to reveal how the GV shell is formed by a helical filament of highly conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder, a site that may act as an elongation center. Subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing structural reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes.

PubMed: 37040766
DOI: 10.1016/j.str.2023.03.011

実験手法

ELECTRON MICROSCOPY (8 Å)