8GAR

Nitrosomonas europaea Cytochrome P460 Arg44Ala

8GAR の概要

• エントリーDOI: 10.2210/pdb8gar/pdb
• 分子名称: Cytochrome P460, HEME C, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: cytochrome, heme protein, metal binding protein
• 由来する生物種: Nitrosomonas europaea
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21914.54

構造登録者

Bollmeyer, M.M.,Lancaster, K.M. (登録日: 2023-02-23, 公開日: 2023-07-05, 最終更新日: 2024-10-30)

主引用文献

Bollmeyer, M.M.,Coleman, R.E.,Majer, S.H.,Ferrao, S.D.,Lancaster, K.M.
Cytochrome P460 Cofactor Maturation Proceeds via Peroxide-Dependent Post-translational Modification.
J.Am.Chem.Soc., 145:14404-14416, 2023

PubMed Abstract: Cytochrome P460s are heme enzymes that oxidize hydroxylamine to nitrous oxide. They bear specialized "heme P460" cofactors that are cross-linked to their host polypeptides by a post-translationally modified lysine residue. Wild-type cytochrome P460 may be isolated as a cross-link-deficient proenzyme following anaerobic overexpression in . When treated with peroxide, this proenzyme undergoes maturation to active enzyme with spectroscopic and catalytic properties that match wild-type cyt P460. This maturation reactivity requires no chaperones─it is intrinsic to the protein. This behavior extends to the broader cytochrome c' superfamily. Accumulated data reveal key contributions from the secondary coordination sphere that enable selective, complete maturation. Spectroscopic data support the intermediacy of a ferryl species along the maturation pathway.

PubMed: 37338957
DOI: 10.1021/jacs.3c03608

実験手法

X-RAY DIFFRACTION (1.55 Å)