8G4G

Crystal Engineering with One 8-mer DNA

8G4G の概要

• エントリーDOI: 10.2210/pdb8g4g/pdb
• 分子名称: DNA (5'-D(P*CP*CP*G)-3'), DNA (5'-D(*AP*TP*CP*GP*GP*CP*CP*G)-3'), DNA (5'-D(*AP*TP*CP*GP*G)-3') (3 entities in total)
• 機能のキーワード: dna, 8-mer, meta-triplex
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 7192.90

構造登録者

Zhao, J.,Zhang, C.,Lu, B.,Seeman, N.C.,Noinaj, N.,Sha, R.,Mao, C. (登録日: 2023-02-09, 公開日: 2023-05-10, 最終更新日: 2024-05-22)

主引用文献

Zhao, J.,Zhang, C.,Lu, B.,Sha, R.,Noinaj, N.,Mao, C.
Divergence and Convergence: Complexity Emerges in Crystal Engineering from an 8-mer DNA.
J.Am.Chem.Soc., 145:10475-10479, 2023

PubMed Abstract: Biology provides plenty of examples on achieving complicated structures out of minimal numbers of building blocks. In contrast, structural complexity of designed molecular systems is achieved by increasing the numbers of component molecules. In this study, the component DNA strand assembles into a highly complex crystal structure via an unusual path of divergence and convergence. This assembly path suggests a route to minimalists for increasing structural complexity. The original purpose of this study is to engineer DNA crystals with high resolution, which is the primary motivation and a key objective for structural DNA nanotechnology. Despite great efforts in the last 40 years, engineered DNA crystals have not yet consistently reached resolution better than 2.5 Å, limiting their potential uses. Our research has shown that small, symmetrical building blocks generally lead to high resolution crystals. Herein, by following this principle, we report an engineered DNA crystal with unprecedented high resolution (2.17 Å) assembled from one single DNA component: an 8-base-long DNA strand. This system has three unique characteristics: (1) It has a very complex architecture, (2) the same DNA strand forms two different structural motifs, both of which are incorporated into the final crystal, and (3) the component DNA molecule is only an 8-base-long DNA strand, which is, arguably, the smallest DNA motif for DNA nanostructures to date. This high resolution opens the possibility of using these DNA crystals to precisely organize guest molecules at the Å level, which could stimulate a range of new investigations.

PubMed: 37134185
DOI: 10.1021/jacs.3c01941

実験手法

X-RAY DIFFRACTION (2.17 Å)