8FZC

HIV-2 Gag Capsid from Immature Virus-like Particles

8FZC の概要

• エントリーDOI: 10.2210/pdb8fzc/pdb
• EMDBエントリー: 29606 29607
• 分子名称: Spacer peptide 2 (1 entity in total)
• 機能のキーワード: retrovirus, lentivirus, gag, capsid, hiv-2, lattice, virus like particle
• 由来する生物種: Human immunodeficiency virus type 2 (ISOLATE ROD)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 75329.47

構造登録者

Talledge, N.,Zhang, W.,Mansky, L.M. (登録日: 2023-01-28, 公開日: 2023-06-07, 最終更新日: 2024-06-19)

主引用文献

Talledge, N.,Yang, H.,Shi, K.,Coray, R.,Yu, G.,Arndt, W.G.,Meng, S.,Baxter, G.C.,Mendonca, L.M.,Castano-Diez, D.,Aihara, H.,Mansky, L.M.,Zhang, W.
HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation.
J.Mol.Biol., 435:168143-168143, 2023

PubMed Abstract: Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used the single particle cryo-electron microscopy method to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag lattice structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation.

PubMed: 37150290
DOI: 10.1016/j.jmb.2023.168143

実験手法

ELECTRON MICROSCOPY (5.5 Å)