8FZB

Crystal structure of human FAM86A

8FZB の概要

• エントリーDOI: 10.2210/pdb8fzb/pdb
• 分子名称: Protein-lysine N-methyltransferase EEF2KMT, S-ADENOSYL-L-HOMOCYSTEINE (2 entities in total)
• 機能のキーワード: protein lysine methyltransferase, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 112550.57

構造登録者

Shao, Z.,Lu, J.,Song, J. (登録日: 2023-01-28, 公開日: 2023-06-21, 最終更新日: 2024-05-22)

主引用文献

Francis, J.W.,Shao, Z.,Narkhede, P.,Trinh, A.T.,Lu, J.,Song, J.,Gozani, O.
The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
J.Biol.Chem., 299:104842-104842, 2023

PubMed Abstract: FAM86A is a class I lysine methyltransferase (KMT) that generates trimethylation on the eukaryotic translation elongation factor 2 (EEF2) at Lys525. Publicly available data from The Cancer Dependency Map project indicate high dependence of hundreds of human cancer cell lines on FAM86A expression. This classifies FAM86A among numerous other KMTs as potential targets for future anticancer therapies. However, selective inhibition of KMTs by small molecules can be challenging due to high conservation within the S-adenosyl methionine (SAM) cofactor binding domain among KMT subfamilies. Therefore, understanding the unique interactions within each KMT-substrate pair can facilitate developing highly specific inhibitors. The FAM86A gene encodes an N-terminal FAM86 domain of unknown function in addition to its C-terminal methyltransferase domain. Here, we used a combination of X-ray crystallography, the AlphaFold algorithms, and experimental biochemistry to identify an essential role of the FAM86 domain in mediating EEF2 methylation by FAM86A. To facilitate our studies, we also generated a selective EEF2K525 methyl antibody. Overall, this is the first report of a biological function for the FAM86 structural domain in any species and an example of a noncatalytic domain participating in protein lysine methylation. The interaction between the FAM86 domain and EEF2 provides a new strategy for developing a specific FAM86A small molecule inhibitor, and our results provide an example in which modeling a protein-protein interaction with AlphaFold expedites experimental biology.

PubMed: 37209825
DOI: 10.1016/j.jbc.2023.104842

実験手法

X-RAY DIFFRACTION (3.35 Å)