8FY4

Structure of NOT1:NOT10:NOT11 module of the chicken CCR4-NOT complex

Summary for 8FY4

• Entry DOI: 10.2210/pdb8fy4/pdb
• EMDB information: 29552
• Descriptor: CCR4-NOT transcription complex subunit 1, CCR4-NOT transcription complex subunit 10, CCR4-NOT transcription complex subunit 11 (3 entities in total)
• Functional Keywords: mrna degradation, gene expression, rna binding protein
• Biological source: Gallus gallus (chicken); More
• Total number of polymer chains: 3
• Total formula weight: 204328.64

Authors

Lea, S.M.,Deme, J.C.,Raisch, T.,Levdansky, Y.,Valkov, E. (deposition date: 2023-01-25, release date: 2023-08-30)

Primary citation

Levdansky, Y.,Raisch, T.,Deme, J.C.,Pekovic, F.,Elmlund, H.,Lea, S.M.,Valkov, E.
Structure and assembly of the NOT10:11 module of the CCR4-NOT complex.
Commun Biol, 6:739-, 2023

PubMed Abstract: NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly.

PubMed: 37460791
DOI: 10.1002/pro.3943

Experimental method

ELECTRON MICROSCOPY (2.57 Å)