8FW8

MtrR from Neisseria gonorrhoeae bound to Progesterone

8FW8 の概要

• エントリーDOI: 10.2210/pdb8fw8/pdb
• 分子名称: HTH-type transcriptional regulator MtrR, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: hth, regulator, hormone, induction, transcription
• 由来する生物種: Neisseria gonorrhoeae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99647.52

構造登録者

Hooks, G.H.,Brennan, R.G. (登録日: 2023-01-20, 公開日: 2024-01-24, 最終更新日: 2024-04-03)

主引用文献

Hooks, G.M.,Ayala, J.C.,Holley, C.L.,Dhulipala, V.,Beggs, G.A.,Perfect, J.R.,Schumacher, M.A.,Shafer, W.M.,Brennan, R.G.
Hormonal steroids induce multidrug resistance and stress response genes in Neisseria gonorrhoeae by binding to MtrR.
Nat Commun, 15:1153-1153, 2024

PubMed Abstract: Transcriptional regulator MtrR inhibits the expression of the multidrug efflux pump operon mtrCDE in the pathogenic bacterium Neisseria gonorrhoeae. Here, we show that MtrR binds the hormonal steroids progesterone, β-estradiol, and testosterone, which are present at urogenital infection sites, as well as ethinyl estrogen, a component of some hormonal contraceptives. Steroid binding leads to the decreased affinity of MtrR for cognate DNA, increased mtrCDE expression, and enhanced antimicrobial resistance. Furthermore, we solve crystal structures of MtrR bound to each steroid, thus revealing their binding mechanisms and the conformational changes that induce MtrR.

PubMed: 38326294
DOI: 10.1038/s41467-024-45195-1

実験手法

X-RAY DIFFRACTION (2.31 Å)