8FVT

Multi-state design of two-state switchable hinge proteins

8FVT の概要

• エントリーDOI: 10.2210/pdb8fvt/pdb
• 分子名称: 3hb12 (1 entity in total)
• 機能のキーワード: de novo protein, metamorphic proteins, switchable hinge proteins, protein dynamics, three-helix bundles
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22386.66

構造登録者

Bera, A.K.,Broerman, A.,Baker, D. (登録日: 2023-01-19, 公開日: 2023-08-16, 最終更新日: 2024-03-06)

主引用文献

Praetorius, F.,Leung, P.J.Y.,Tessmer, M.H.,Broerman, A.,Demakis, C.,Dishman, A.F.,Pillai, A.,Idris, A.,Juergens, D.,Dauparas, J.,Li, X.,Levine, P.M.,Lamb, M.,Ballard, R.K.,Gerben, S.R.,Nguyen, H.,Kang, A.,Sankaran, B.,Bera, A.K.,Volkman, B.F.,Nivala, J.,Stoll, S.,Baker, D.
Design of stimulus-responsive two-state hinge proteins.
Science, 381:754-760, 2023

PubMed Abstract: In nature, proteins that switch between two conformations in response to environmental stimuli structurally transduce biochemical information in a manner analogous to how transistors control information flow in computing devices. Designing proteins with two distinct but fully structured conformations is a challenge for protein design as it requires sculpting an energy landscape with two distinct minima. Here we describe the design of "hinge" proteins that populate one designed state in the absence of ligand and a second designed state in the presence of ligand. X-ray crystallography, electron microscopy, double electron-electron resonance spectroscopy, and binding measurements demonstrate that despite the significant structural differences the two states are designed with atomic level accuracy and that the conformational and binding equilibria are closely coupled.

PubMed: 37590357
DOI: 10.1126/science.adg7731

実験手法

X-RAY DIFFRACTION (3.07 Å)