8FUL

Heterologous AibH1H2 purified from Lysogeny broth

8FUL の概要

• エントリーDOI: 10.2210/pdb8ful/pdb
• 分子名称: Amidohydrolase, FE (III) ION, GLYCEROL, ... (7 entities in total)
• 機能のキーワード: alpha aminoisobutyric acid hydroxylase amidohydrolase-like dimetallic, oxidoreductase
• 由来する生物種: Rhodococcus wratislaviensis NBRC 100605; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 345967.89

構造登録者

Powell, M.M.,Rittle, J. (登録日: 2023-01-17, 公開日: 2023-04-05, 最終更新日: 2024-03-20)

主引用文献

Powell, M.M.,Rao, G.,Britt, R.D.,Rittle, J.
Enzymatic Hydroxylation of Aliphatic C-H Bonds by a Mn/Fe Cofactor.
J.Am.Chem.Soc., 145:16526-16537, 2023

PubMed Abstract: The aerobic oxidation of carbon-hydrogen (C-H) bonds in biology is currently known to be accomplished by a limited set of cofactors that typically include heme, nonheme iron, and copper. While manganese cofactors perform difficult oxidation reactions, including water oxidation within Photosystem II, they are generally not known to be used for C-H bond activation, and those that do catalyze this important reaction display limited intrinsic reactivity. Here we report that the 2-aminoisobutyric acid hydroxylase from , AibH1H2, requires manganese to functionalize a strong, aliphatic C-H bond (BDE = 100 kcal/mol). Structural and spectroscopic studies of this enzyme reveal a redox-active, heterobimetallic manganese-iron active site at the locus of O activation and substrate coordination. This result expands the known reactivity of biological manganese-iron cofactors, which was previously restricted to single-electron transfer or stoichiometric protein oxidation. Furthermore, the AibH1H2 cofactor is supported by a protein fold distinct from typical bimetallic oxygenases, and bioinformatic analyses identify related proteins abundant in microorganisms. This suggests that many uncharacterized monooxygenases may similarly require manganese to perform oxidative biochemical tasks.

PubMed: 37471626
DOI: 10.1021/jacs.3c03419

実験手法

X-RAY DIFFRACTION (2.29 Å)