8FTD

Structure of Escherichia coli CedA in complex with transcription initiation complex

8FTD の概要

• エントリーDOI: 10.2210/pdb8ftd/pdb
• EMDBエントリー: 29423
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, ZINC ION, ... (11 entities in total)
• 機能のキーワード: escherichia coli, ceda, initiation complex, transcription, transferase-dna complex, transferase/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 559830.07

構造登録者

Liu, M.,Vassyliev, N.,Nudler, E. (登録日: 2023-01-11, 公開日: 2024-01-10, 最終更新日: 2025-06-04)

主引用文献

Vasilyev, N.,Liu, M.M.J.,Epshtein, V.,Shamovsky, I.,Nudler, E.
General transcription factor from Escherichia coli with a distinct mechanism of action.
Nat.Struct.Mol.Biol., 31:141-149, 2024

PubMed Abstract: Gene expression in Escherichia coli is controlled by well-established mechanisms that activate or repress transcription. Here, we identify CedA as an unconventional transcription factor specifically associated with the RNA polymerase (RNAP) σ holoenzyme. Structural and biochemical analysis of CedA bound to RNAP reveal that it bridges distant domains of β and σ subunits to stabilize an open-promoter complex. CedA does so without contacting DNA. We further show that cedA is strongly induced in response to amino acid starvation, oxidative stress and aminoglycosides. CedA provides a basal level of tolerance to these clinically relevant antibiotics, as well as to rifampicin and peroxide. Finally, we show that CedA modulates transcription of hundreds of bacterial genes, which explains its pleotropic effect on cell physiology and pathogenesis.

PubMed: 38177674
DOI: 10.1038/s41594-023-01154-w

実験手法

ELECTRON MICROSCOPY (2.76 Å)