8FFY

Cryo-electron microscopy structure of human mt-SerRS in complex with mt-tRNA(UGA-TL)

8FFY の概要

• エントリーDOI: 10.2210/pdb8ffy/pdb
• EMDBエントリー: 29070
• 分子名称: Serine--tRNA ligase, mitochondrial, mt-tRNA(UGA-TL), 5'-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE (3 entities in total)
• 機能のキーワード: trna, serrs, transcription, ligase-rna complex, ligase/rna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 138994.99

構造登録者

Hirschi, M.,Kuhle, B.,Doerfel, L.,Schimmel, P.,Lander, G. (登録日: 2022-12-11, 公開日: 2023-08-23)

主引用文献

Kuhle, B.,Hirschi, M.,Doerfel, L.K.,Lander, G.C.,Schimmel, P.
Structural basis for a degenerate tRNA identity code and the evolution of bimodal specificity in human mitochondrial tRNA recognition.
Nat Commun, 14:4794-4794, 2023

PubMed Abstract: Animal mitochondrial gene expression relies on specific interactions between nuclear-encoded aminoacyl-tRNA synthetases and mitochondria-encoded tRNAs. Their evolution involves an antagonistic interplay between strong mutation pressure on mtRNAs and selection pressure to maintain their essential function. To understand the molecular consequences of this interplay, we analyze the human mitochondrial serylation system, in which one synthetase charges two highly divergent mtRNA isoacceptors. We present the cryo-EM structure of human mSerRS in complex with mtRNA, and perform a structural and functional comparison with the mSerRS-mtRNA complex. We find that despite their common function, mtRNA and mtRNA show no constrain to converge on shared structural or sequence identity motifs for recognition by mSerRS. Instead, mSerRS evolved a bimodal readout mechanism, whereby a single protein surface recognizes degenerate identity features specific to each mtRNA. Our results show how the mutational erosion of mtRNAs drove a remarkable innovation of intermolecular specificity rules, with multiple evolutionary pathways leading to functionally equivalent outcomes.

PubMed: 37558671
DOI: 10.1038/s41467-023-40354-2

実験手法

ELECTRON MICROSCOPY (3.6 Å)