8FFN

RR-bound wildtype rabbit TRPV5 in nanodiscs

8FFN の概要

• エントリーDOI: 10.2210/pdb8ffn/pdb
• EMDBエントリー: 29048
• 分子名称: Transient receptor potential cation channel subfamily V member 5, ruthenium(6+) azanide pentaamino(oxido)ruthenium (1/4/2), (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, ... (4 entities in total)
• 機能のキーワード: trpv2, trpv5, trp channel, ruthenium red, membrane protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 344951.66

構造登録者

Fluck, E.C.,De Jesus-Perez, J.J.,Pumroy, R.A.,Protopopova, A.D.,Rocereta, J.A.,Moiseenkova-Bell, V.Y. (登録日: 2022-12-09, 公開日: 2024-02-07, 最終更新日: 2024-02-21)

主引用文献

Pumroy, R.A.,De Jesus-Perez, J.J.,Protopopova, A.D.,Rocereta, J.A.,Fluck, E.C.,Fricke, T.,Lee, B.H.,Rohacs, T.,Leffler, A.,Moiseenkova-Bell, V.
Molecular details of ruthenium red pore block in TRPV channels.
Embo Rep., 25:506-523, 2024

PubMed Abstract: Transient receptor potential vanilloid (TRPV) channels play a critical role in calcium homeostasis, pain sensation, immunological response, and cancer progression. TRPV channels are blocked by ruthenium red (RR), a universal pore blocker for a wide array of cation channels. Here we use cryo-electron microscopy to reveal the molecular details of RR block in TRPV2 and TRPV5, members of the two TRPV subfamilies. In TRPV2 activated by 2-aminoethoxydiphenyl borate, RR is tightly coordinated in the open selectivity filter, blocking ion flow and preventing channel inactivation. In TRPV5 activated by phosphatidylinositol 4,5-bisphosphate, RR blocks the selectivity filter and closes the lower gate through an interaction with polar residues in the pore vestibule. Together, our results provide a detailed understanding of TRPV subfamily pore block, the dynamic nature of the selectivity filter and allosteric communication between the selectivity filter and lower gate.

PubMed: 38225355
DOI: 10.1038/s44319-023-00050-0

実験手法

ELECTRON MICROSCOPY (2.96 Å)