8FBD

Crystal structure of OrfX1-OrfX3 complex from Clostridium botulinum E1

これはPDB形式変換不可エントリーです。

8FBD の概要

• エントリーDOI: 10.2210/pdb8fbd/pdb
• 分子名称: Neurotoxin complex component Orf-X1, Neurotoxin complex component Orf-X3, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: botulinum neurotoxin, unknown function
• 由来する生物種: Clostridium botulinum E1; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 142567.99

構造登録者

Liu, S.,Gao, L. (登録日: 2022-11-29, 公開日: 2022-12-21, 最終更新日: 2024-05-22)

主引用文献

Gao, L.,Lam, K.H.,Liu, S.,Przykopanski, A.,Lubke, J.,Qi, R.,Kruger, M.,Nowakowska, M.B.,Selby, K.,Douillard, F.P.,Dorner, M.B.,Perry, K.,Lindstrom, M.,Dorner, B.G.,Rummel, A.,Jin, R.
Crystal structures of OrfX1, OrfX2 and the OrfX1-OrfX3 complex from the orfX gene cluster of botulinum neurotoxin E1.
Febs Lett., 597:524-537, 2023

PubMed Abstract: Botulinum neurotoxins (BoNTs) are among the most lethal toxins known to humans, comprising seven established serotypes termed BoNT/A-G encoded in two types of gene clusters (ha and orfX) in BoNT-producing clostridia. The ha cluster encodes four non-toxic neurotoxin-associated proteins (NAPs) that assemble with BoNTs to protect and enhance their oral toxicity. However, the structure and function of the orfX-type NAPs remain largely unknown. Here, we report the crystal structures for OrfX1, OrfX2, and an OrfX1-OrfX3 complex, which are encoded in the orfX cluster of a BoNT/E1-producing Clostridium botulinum strain associated with human foodborne botulism. These structures lay the foundation for future studies on the potential roles of OrfX proteins in oral intoxication and pathogenesis of BoNTs.

PubMed: 36653893
DOI: 10.1002/1873-3468.14576

実験手法

X-RAY DIFFRACTION (2.05 Å)