8F9Y

SAL1 from Arabidopsis thaliana

「5ESY」から置き換えられました

8F9Y の概要

• エントリーDOI: 10.2210/pdb8f9y/pdb
• 分子名称: SAL1 phosphatase, MAGNESIUM ION, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, sal1
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37708.42

構造登録者

Frkic, R.L.,Kaczmarski, J.A.,Tan, L.,Jackson, C.J. (登録日: 2022-11-24, 公開日: 2023-01-18, 最終更新日: 2024-05-22)

主引用文献

Chan, K.X.,Mabbitt, P.D.,Phua, S.Y.,Mueller, J.W.,Nisar, N.,Gigolashvili, T.,Stroeher, E.,Grassl, J.,Arlt, W.,Estavillo, G.M.,Jackson, C.J.,Pogson, B.J.
Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase.
Proc.Natl.Acad.Sci.USA, 113:E4567-E4576, 2016

PubMed Abstract: Intracellular signaling during oxidative stress is complex, with organelle-to-nucleus retrograde communication pathways ill-defined or incomplete. Here we identify the 3'-phosphoadenosine 5'-phosphate (PAP) phosphatase SAL1 as a previously unidentified and conserved oxidative stress sensor in plant chloroplasts. Arabidopsis thaliana SAL1 (AtSAL1) senses changes in photosynthetic redox poise, hydrogen peroxide, and superoxide concentrations in chloroplasts via redox regulatory mechanisms. AtSAL1 phosphatase activity is suppressed by dimerization, intramolecular disulfide formation, and glutathionylation, allowing accumulation of its substrate, PAP, a chloroplast stress retrograde signal that regulates expression of plastid redox associated nuclear genes (PRANGs). This redox regulation of SAL1 for activation of chloroplast signaling is conserved in the plant kingdom, and the plant protein has evolved enhanced redox sensitivity compared with its yeast ortholog. Our results indicate that in addition to sulfur metabolism, SAL1 orthologs have evolved secondary functions in oxidative stress sensing in the plant kingdom.

PubMed: 27432987
DOI: 10.1073/pnas.1604936113

実験手法

X-RAY DIFFRACTION (2.6 Å)