8F5Z

Composite map of CryoEM structure of Arabidopsis thaliana phytochrome A

8F5Z の概要

• エントリーDOI: 10.2210/pdb8f5z/pdb
• EMDBエントリー: 28869 28870 28871 28872
• 分子名称: Phytochrome A, 3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid (2 entities in total)
• 機能のキーワード: phytochrome, asymmetric dimer, gene regulation
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 250593.00

構造登録者

Li, H.,Li, H. (登録日: 2022-11-15, 公開日: 2023-06-28, 最終更新日: 2024-10-23)

主引用文献

Burgie, E.S.,Li, H.,Gannam, Z.T.K.,McLoughlin, K.E.,Vierstra, R.D.,Li, H.
The structure of Arabidopsis phytochrome A reveals topological and functional diversification among the plant photoreceptor isoforms.
Nat.Plants, 9:1116-1129, 2023

PubMed Abstract: Plants employ a divergent cohort of phytochrome (Phy) photoreceptors to govern many aspects of morphogenesis through reversible photointerconversion between inactive Pr and active Pfr conformers. The two most influential are PhyA whose retention of Pfr enables sensation of dim light, while the relative instability of Pfr for PhyB makes it better suited for detecting full sun and temperature. To better understand these contrasts, we solved, by cryo-electron microscopy, the three-dimensional structure of full-length PhyA as Pr. Like PhyB, PhyA dimerizes through head-to-head assembly of its C-terminal histidine kinase-related domains (HKRDs), while the remainder assembles as a head-to-tail light-responsive platform. Whereas the platform and HKRDs associate asymmetrically in PhyB dimers, these lopsided connections are absent in PhyA. Analysis of truncation and site-directed mutants revealed that this decoupling and altered platform assembly have functional consequences for Pfr stability of PhyA and highlights how plant Phy structural diversification has extended light and temperature perception.

PubMed: 37291396
DOI: 10.1038/s41477-023-01435-8

実験手法

ELECTRON MICROSCOPY (3.2 Å)