8F5T

Rabbit muscle pyruvate kinase in complex with sodium and magnesium

8F5T の概要

• エントリーDOI: 10.2210/pdb8f5t/pdb
• 分子名称: Pyruvate kinase PKM, MAGNESIUM ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: glycolysis, metabolic kinase, central metabolism, cytosolic protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 466299.63

構造登録者

Holyoak, T.,Fenton, A.W. (登録日: 2022-11-15, 公開日: 2023-05-24, 最終更新日: 2023-10-25)

主引用文献

Swint-Kruse, L.,Dougherty, L.L.,Page, B.,Wu, T.,O'Neil, P.T.,Prasannan, C.B.,Timmons, C.,Tang, Q.,Parente, D.J.,Sreenivasan, S.,Holyoak, T.,Fenton, A.W.
PYK-SubstitutionOME: an integrated database containing allosteric coupling, ligand affinity and mutational, structural, pathological, bioinformatic and computational information about pyruvate kinase isozymes.
Database (Oxford), 2023:-, 2023

PubMed Abstract: Interpreting changes in patient genomes, understanding how viruses evolve and engineering novel protein function all depend on accurately predicting the functional outcomes that arise from amino acid substitutions. To that end, the development of first-generation prediction algorithms was guided by historic experimental datasets. However, these datasets were heavily biased toward substitutions at positions that have not changed much throughout evolution (i.e. conserved). Although newer datasets include substitutions at positions that span a range of evolutionary conservation scores, these data are largely derived from assays that agglomerate multiple aspects of function. To facilitate predictions from the foundational chemical properties of proteins, large substitution databases with biochemical characterizations of function are needed. We report here a database derived from mutational, biochemical, bioinformatic, structural, pathological and computational studies of a highly studied protein family-pyruvate kinase (PYK). A centerpiece of this database is the biochemical characterization-including quantitative evaluation of allosteric regulation-of the changes that accompany substitutions at positions that sample the full conservation range observed in the PYK family. We have used these data to facilitate critical advances in the foundational studies of allosteric regulation and protein evolution and as rigorous benchmarks for testing protein predictions. We trust that the collected dataset will be useful for the broader scientific community in the further development of prediction algorithms. Database URL https://github.com/djparente/PYK-DB.

PubMed: 37171062
DOI: 10.1093/database/baad030

実験手法

X-RAY DIFFRACTION (2.41 Å)