8F5Q

Crystal structure of human PCNA in complex with the PIP box of FBH1

8F5Q の概要

• エントリーDOI: 10.2210/pdb8f5q/pdb
• 分子名称: Proliferating cell nuclear antigen, F-box DNA helicase 1 (3 entities in total)
• 機能のキーワード: complex, dna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89336.74

構造登録者

Liu, J.,Chaves-Arquero, B.,Wei, P.,Tencer, H.,Zhang, G.,Blanco, F.,Kutateladze, T. (登録日: 2022-11-15, 公開日: 2023-09-27, 最終更新日: 2026-03-04)

主引用文献

Liu, J.,Chaves-Arquero, B.,Wei, P.,Tencer, A.H.,Ruiz-Albor, A.,Zhang, G.,Blanco, F.J.,Kutateladze, T.G.
Molecular insight into the PCNA-binding mode of FBH1.
Structure, 31:511-517.e3, 2023

PubMed Abstract: F-box DNA helicase 1 (FBH1) is involved in the regulation of cell responses to replicative stress. FBH1 is recruited to stalled DNA replication fork by PCNA where it inhibits homologous recombination and catalyzes fork regression. Here, we report the structural basis for the molecular recognition of two distinctly different motifs of FBH1, FBH1 and FBH1, by PCNA. The crystal structure of PCNA in complex with FBH1 and analysis of NMR perturbations reveal overlapped FBH1 and FBH1 binding sites of PCNA and the dominant contribution of FBH1 in this interaction.

PubMed: 36990095
DOI: 10.1016/j.str.2023.03.004

実験手法

X-RAY DIFFRACTION (1.9 Å)