8F4K

RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution

8F4K の概要

• エントリーDOI: 10.2210/pdb8f4k/pdb
• 分子名称: Photosystem II protein D1 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (37 entities in total)
• 機能のキーワード: water oxidation, oec, manganese cluster, photosynthesis
• 由来する生物種: Thermosynechococcus vestitus BP-1; 詳細
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 758862.36

構造登録者

Bhowmick, A.,Hussein, R.,Bogacz, I.,Simon, P.S.,Ibrahim, M.,Chatterjee, R.,Doyle, M.D.,Cheah, M.H.,Fransson, T.,Chernev, P.,Kim, I.-S.,Makita, H.,Dasgupta, M.,Kaminsky, C.J.,Zhang, M.,Gatcke, J.,Haupt, S.,Nangca, I.I.,Keable, S.M.,Aydin, O.,Tono, K.,Owada, S.,Gee, L.B.,Fuller, F.D.,Batyuk, A.,Alonso-Mori, R.,Holton, J.M.,Paley, D.W.,Moriarty, N.W.,Mamedov, F.,Adams, P.D.,Brewster, A.S.,Dobbek, H.,Sauter, N.K.,Bergmann, U.,Zouni, A.,Messinger, J.,Kern, J.,Yano, J.,Yachandra, V.K. (登録日: 2022-11-10, 公開日: 2023-03-22, 最終更新日: 2023-10-25)

主引用文献

Bhowmick, A.,Hussein, R.,Bogacz, I.,Simon, P.S.,Ibrahim, M.,Chatterjee, R.,Doyle, M.D.,Cheah, M.H.,Fransson, T.,Chernev, P.,Kim, I.S.,Makita, H.,Dasgupta, M.,Kaminsky, C.J.,Zhang, M.,Gatcke, J.,Haupt, S.,Nangca, I.I.,Keable, S.M.,Aydin, A.O.,Tono, K.,Owada, S.,Gee, L.B.,Fuller, F.D.,Batyuk, A.,Alonso-Mori, R.,Holton, J.M.,Paley, D.W.,Moriarty, N.W.,Mamedov, F.,Adams, P.D.,Brewster, A.S.,Dobbek, H.,Sauter, N.K.,Bergmann, U.,Zouni, A.,Messinger, J.,Kern, J.,Yano, J.,Yachandra, V.K.
Structural evidence for intermediates during O 2 formation in photosystem II.
Nature, 617:629-636, 2023

PubMed Abstract: In natural photosynthesis, the light-driven splitting of water into electrons, protons and molecular oxygen forms the first step of the solar-to-chemical energy conversion process. The reaction takes place in photosystem II, where the MnCaO cluster first stores four oxidizing equivalents, the S to S intermediate states in the Kok cycle, sequentially generated by photochemical charge separations in the reaction center and then catalyzes the O-O bond formation chemistry. Here, we report room temperature snapshots by serial femtosecond X-ray crystallography to provide structural insights into the final reaction step of Kok's photosynthetic water oxidation cycle, the S→[S]→S transition where O is formed and Kok's water oxidation clock is reset. Our data reveal a complex sequence of events, which occur over micro- to milliseconds, comprising changes at the MnCaO cluster, its ligands and water pathways as well as controlled proton release through the hydrogen-bonding network of the Cl1 channel. Importantly, the extra O atom O, which was introduced as a bridging ligand between Ca and Mn1 during the S→S transition, disappears or relocates in parallel with Y reduction starting at approximately 700 μs after the third flash. The onset of O evolution, as indicated by the shortening of the Mn1-Mn4 distance, occurs at around 1,200 μs, signifying the presence of a reduced intermediate, possibly a bound peroxide.

PubMed: 37138085
DOI: 10.1038/s41586-023-06038-z

実験手法

X-RAY DIFFRACTION (2.16 Å)