8F43
HNH Nuclease Domain from G. stearothermophilus Cas9, K597A mutant
Summary for 8F43
| Entry DOI | 10.2210/pdb8f43/pdb |
| Related | 7MPZ |
| Descriptor | CRISPR-associated endonuclease Cas9 (2 entities in total) |
| Functional Keywords | nuclease domain, crispr cas9, dna binding protein, hydrolase |
| Biological source | Geobacillus stearothermophilus |
| Total number of polymer chains | 1 |
| Total formula weight | 13020.69 |
| Authors | D'Ordine, A.M.,Belato, H.B.,Lisi, G.P.,Jogl, G. (deposition date: 2022-11-10, release date: 2022-12-21, Last modification date: 2024-05-01) |
| Primary citation | Belato, H.B.,Norbrun, C.,Luo, J.,Pindi, C.,Sinha, S.,D'Ordine, A.M.,Jogl, G.,Palermo, G.,Lisi, G.P. Disruption of electrostatic contacts in the HNH nuclease from a thermophilic Cas9 rewires allosteric motions and enhances high-temperature DNA cleavage. J.Chem.Phys., 157:225103-225103, 2022 Cited by PubMed: 36546784DOI: 10.1063/5.0128815 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.37 Å) |
Structure validation
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