8F2G

Crystal structure of Hen Egg White Lysozyme at 0.44 GPa

Summary for 8F2G

• Entry DOI: 10.2210/pdb8f2g/pdb
• Descriptor: Lysozyme C (2 entities in total)
• Functional Keywords: high pressure structure, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 14331.16

Authors

Marshall, A.C.,Boer, S.A.,Turner, G.,Moggach, S.A.,Bond, C.S.,Vrielink, A. (deposition date: 2022-11-08, release date: 2022-12-07, Last modification date: 2024-11-06)

Primary citation

Boer, S.A.,Price, J.R.,Riboldi-Tunnicliffe, A.,Williamson, R.,Rostan, R.,Summers, A.,Turner, G.F.,Jones, I.,Bond, C.S.,Vrielink, A.,Marshall, A.C.,Hitchings, J.,Moggach, S.A.
High-pressure single-crystal diffraction at the Australian Synchrotron.
J.Synchrotron Radiat., 30:841-846, 2023

PubMed Abstract: A new high-pressure single-crystal diffraction setup has been designed and implemented at the Australian Synchrotron for collecting molecular and protein crystal structures. The setup incorporates a modified micro-Merrill-Bassett cell and holder designed specifically to fit onto the horizontal air-bearing goniometer, allowing high-pressure diffraction measurements to be collected with little to no modification of the beamline setup compared with ambient data collections. Compression data for the amino acid, L-threonine, and the protein, hen egg-white lysozyme, were collected, showcasing the capabilities of the setup.

PubMed: 37318368
DOI: 10.1107/S160057752300406X

Experimental method

X-RAY DIFFRACTION (1.84 Å)