8F0X

Cryo-EM structure of Kap114 bound to H2A-H2B

8F0X の概要

• エントリーDOI: 10.2210/pdb8f0x/pdb
• 関連するPDBエントリー: 6N1Z
• EMDBエントリー: 28782 28788 28796
• 分子名称: Importin subunit beta-5, Histone H2A.2, Histone H2B.2 (3 entities in total)
• 機能のキーワード: karyopherin beta nuclear transport histone histone chaperone, protein transport-structural protein complex, protein transport/structural protein
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 142034.82

構造登録者

Jiou, J.,Chook, Y.M. (登録日: 2022-11-04, 公開日: 2023-07-26, 最終更新日: 2025-05-21)

主引用文献

Jiou, J.,Shaffer, J.M.,Bernades, N.E.,Fung, H.Y.J.,Kikumoto Dias, J.,D'Arcy, S.,Chook, Y.M.
Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex.
Proc.Natl.Acad.Sci.USA, 120:e2301199120-e2301199120, 2023

PubMed Abstract: Previously, we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import systems where RanGTP dissociates cargoes from their importins, RanGTP binds stably to the Importin-9•H2A-H2B complex, and formation of the ternary RanGTP•Importin-9•H2A-H2B complex facilitates H2A-H2B release to the assembling nucleosome. It was unclear how RanGTP and the cargo H2A-H2B can bind simultaneously to an importin, and how interactions of the three components position H2A-H2B for release. Here, we show cryo-EM structures of Importin-9•RanGTP and of its yeast homolog Kap114, including Kap114•RanGTP, Kap114•H2A-H2B, and RanGTP•Kap114•H2A-H2B, to explain how the conserved Kap114 binds H2A-H2B and RanGTP simultaneously and how the GTPase primes histone transfer to the nucleosome. In the ternary complex, RanGTP binds to the N-terminal repeats of Kap114 in the same manner as in the Kap114/Importin-9•RanGTP complex, and H2A-H2B binds via its acidic patch to the Kap114 C-terminal repeats much like in the Kap114/Importin-9•H2A-H2B complex. Ran binds to a different conformation of Kap114 in the ternary RanGTP•Kap114•H2A-H2B complex. Here, Kap114 no longer contacts the H2A-H2B surface proximal to the H2A docking domain that drives nucleosome assembly, positioning it for transfer to the assembling nucleosome or to dedicated H2A-H2B chaperones in the nucleus.

PubMed: 37450495
DOI: 10.1073/pnas.2301199120

実験手法

ELECTRON MICROSCOPY (3.21 Å)