8F0I

Crystal structure of SARS-CoV-2 receptor binding domain in complex with human antibody COVA309-22

8F0I の概要

• エントリーDOI: 10.2210/pdb8f0i/pdb
• 分子名称: Spike protein S1, COVA309-22 heavy chain, COVA309-22 light chain, ... (4 entities in total)
• 機能のキーワード: antibody, sars-cov-2, coronavirus, rbd, covid-19, immune system, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 212441.69

構造登録者

Yuan, M.,Wilson, I.A. (登録日: 2022-11-03, 公開日: 2023-09-13, 最終更新日: 2023-11-01)

主引用文献

Guerra, D.,Beaumont, T.,Radic, L.,Kerster, G.,van der Straten, K.,Yuan, M.,Torres, J.L.,Lee, W.H.,Liu, H.,Poniman, M.,Bontjer, I.,Burger, J.A.,Claireaux, M.,Caniels, T.G.,Snitselaar, J.L.,Bijl, T.P.L.,Kruijer, S.,Ozorowski, G.,Gideonse, D.,Sliepen, K.,Ward, A.B.,Eggink, D.,de Bree, G.J.,Wilson, I.A.,Sanders, R.W.,van Gils, M.J.
Broad SARS-CoV-2 neutralization by monoclonal and bispecific antibodies derived from a Gamma-infected individual.
Iscience, 26:108009-108009, 2023

PubMed Abstract: The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has remained a medical threat due to the evolution of multiple variants that acquire resistance to vaccines and prior infection. Therefore, it is imperative to discover monoclonal antibodies (mAbs) that neutralize a broad range of SARS-CoV-2 variants. A stabilized spike glycoprotein was used to enrich antigen-specific B cells from an individual with a primary Gamma variant infection. Five mAbs selected from those B cells showed considerable neutralizing potency against multiple variants, with COVA309-35 being the most potent against the autologous virus, as well as Omicron BA.1 and BA.2, and COVA309-22 having binding and neutralization activity against Omicron BA.4/5, BQ.1.1, and XBB.1. When combining the COVA309 mAbs as cocktails or bispecific antibodies, the breadth and potency were improved. In addition, the mechanism of cross-neutralization of the COVA309 mAbs was elucidated by structural analysis. Altogether these data indicate that a Gamma-infected individual can develop broadly neutralizing antibodies.

PubMed: 37841584
DOI: 10.1016/j.isci.2023.108009

実験手法

X-RAY DIFFRACTION (3.7 Å)