8EU9

Class1 of the INO80-Nucleosome complex

これはPDB形式変換不可エントリーです。

8EU9 の概要

• エントリーDOI: 10.2210/pdb8eu9/pdb
• EMDBエントリー: 28609
• 分子名称: Chromatin-remodeling ATPase INO80, Actin-related protein 5, Chromatin-remodeling complex subunit IES6, ... (7 entities in total)
• 機能のキーワード: chromatin remodeler, hexasome, dna binding protein, dna binding protein-hydrolase complex, dna binding protein/hydrolase
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 457862.21

構造登録者

Wu, H.,Munoz, E.,Gourdet, M.,Cheng, Y.F.,Narlikar, G. (登録日: 2022-10-18, 公開日: 2023-07-12, 最終更新日: 2024-06-19)

主引用文献

Wu, H.,Munoz, E.N.,Hsieh, L.J.,Chio, U.S.,Gourdet, M.A.,Narlikar, G.J.,Cheng, Y.
Reorientation of INO80 on hexasomes reveals basis for mechanistic versatility.
Science, 381:319-324, 2023

PubMed Abstract: Unlike other chromatin remodelers, INO80 preferentially mobilizes hexasomes, which can form during transcription. Why INO80 prefers hexasomes over nucleosomes remains unclear. Here, we report structures of INO80 bound to a hexasome or a nucleosome. INO80 binds the two substrates in substantially different orientations. On a hexasome, INO80 places its ATPase subunit, Ino80, at superhelical location -2 (SHL -2), in contrast to SHL -6 and SHL -7, as previously seen on nucleosomes. Our results suggest that INO80 action on hexasomes resembles action by other remodelers on nucleosomes such that Ino80 is maximally active near SHL -2. The SHL -2 position also plays a critical role for nucleosome remodeling by INO80. Overall, the mechanistic adaptations used by INO80 for preferential hexasome sliding imply that subnucleosomal particles play considerable regulatory roles.

PubMed: 37384669
DOI: 10.1126/science.adf4197

実験手法

ELECTRON MICROSCOPY (3.48 Å)