8ERC

Human Membrane-bound O-acyltransferase 7

8ERC の概要

• エントリーDOI: 10.2210/pdb8erc/pdb
• EMDBエントリー: 28552
• 分子名称: Lysophospholipid acyltransferase 7 (1 entity in total)
• 機能のキーワード: lipid metabolism membrane remodeling, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54196.28

構造登録者

Wang, K.,Liao, M.,Farese, R.V.,Walther, T.C. (登録日: 2022-10-11, 公開日: 2023-09-27, 最終更新日: 2025-06-04)

主引用文献

Wang, K.,Lee, C.W.,Sui, X.,Kim, S.,Wang, S.,Higgs, A.B.,Baublis, A.J.,Voth, G.A.,Liao, M.,Walther, T.C.,Farese Jr., R.V.
The structure of phosphatidylinositol remodeling MBOAT7 reveals its catalytic mechanism and enables inhibitor identification.
Nat Commun, 14:3533-3533, 2023

PubMed Abstract: Cells remodel glycerophospholipid acyl chains via the Lands cycle to adjust membrane properties. Membrane-bound O-acyltransferase (MBOAT) 7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA. MBOAT7 mutations cause brain developmental disorders, and reduced expression is linked to fatty liver disease. In contrast, increased MBOAT7 expression is linked to hepatocellular and renal cancers. The mechanistic basis of MBOAT7 catalysis and substrate selectivity are unknown. Here, we report the structure and a model for the catalytic mechanism of human MBOAT7. Arachidonyl-CoA and lyso-PI access the catalytic center through a twisted tunnel from the cytosol and lumenal sides, respectively. N-terminal residues on the ER lumenal side determine phospholipid headgroup selectivity: swapping them between MBOATs 1, 5, and 7 converts enzyme specificity for different lyso-phospholipids. Finally, the MBOAT7 structure and virtual screening enabled identification of small-molecule inhibitors that may serve as lead compounds for pharmacologic development.

PubMed: 37316513
DOI: 10.1038/s41467-023-38932-5

実験手法

ELECTRON MICROSCOPY (3.7 Å)