8EP9

The capsid structure of Human Parvovirus 4

This is a non-PDB format compatible entry.

Summary for 8EP9

• Entry DOI: 10.2210/pdb8ep9/pdb
• EMDB information: 28522
• Descriptor: Human Parvovirus 4 (1 entity in total)
• Functional Keywords: parvovirus, capsid, parv4, tetraparvovirus, pathogen, virus like particle
• Biological source: Human parvovirus 4
• Total number of polymer chains: 60
• Total formula weight: 3701449.68

Authors

Mietzsch, M.,McKenna, R. (deposition date: 2022-10-05, release date: 2022-11-09, Last modification date: 2024-06-19)

Primary citation

Lakshmanan, R.,Mietzsch, M.,Jimenez Ybargollin, A.,Chipman, P.,Fu, X.,Qiu, J.,Soderlund-Venermo, M.,McKenna, R.
Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait.
Viruses, 14:-, 2022

PubMed Abstract: Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera and , respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10-30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics.

PubMed: 36298773
DOI: 10.3390/v14102219

Experimental method

ELECTRON MICROSCOPY (3.12 Å)