8EOZ

Precisely patterned nanofibers made from extendable protein multiplexes

8EOZ の概要

• エントリーDOI: 10.2210/pdb8eoz/pdb
• 分子名称: C3HR3_4r (1 entity in total)
• 機能のキーワード: de novo design, nanofibers, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28256.77

構造登録者

Bera, A.K.,Bethel, N.P.,Kang, A. (登録日: 2022-10-04, 公開日: 2023-08-16, 最終更新日: 2023-12-20)

主引用文献

Bethel, N.P.,Borst, A.J.,Parmeggiani, F.,Bick, M.J.,Brunette, T.J.,Nguyen, H.,Kang, A.,Bera, A.K.,Carter, L.,Miranda, M.C.,Kibler, R.D.,Lamb, M.,Li, X.,Sankaran, B.,Baker, D.
Precisely patterned nanofibres made from extendable protein multiplexes.
Nat.Chem., 15:1664-1671, 2023

PubMed Abstract: Molecular systems with coincident cyclic and superhelical symmetry axes have considerable advantages for materials design as they can be readily lengthened or shortened by changing the length of the constituent monomers. Among proteins, alpha-helical coiled coils have such symmetric, extendable architectures, but are limited by the relatively fixed geometry and flexibility of the helical protomers. Here we describe a systematic approach to generating modular and rigid repeat protein oligomers with coincident C to C and superhelical symmetry axes that can be readily extended by repeat propagation. From these building blocks, we demonstrate that a wide range of unbounded fibres can be systematically designed by introducing hydrophilic surface patches that force staggering of the monomers; the geometry of such fibres can be precisely tuned by varying the number of repeat units in the monomer and the placement of the hydrophilic patches.

PubMed: 37667012
DOI: 10.1038/s41557-023-01314-x

実験手法

X-RAY DIFFRACTION (3 Å)