8EON の概要
| エントリーDOI | 10.2210/pdb8eon/pdb |
| EMDBエントリー | 28405 29406 |
| 分子名称 | Baseplate component gp33, Baseplate component gp34, Baseplate component gp36, ... (9 entities in total) |
| 機能のキーワード | pseudomonas, phage, baseplate, virus |
| 由来する生物種 | Pseudomonas phage vB_PaeM_E217 詳細 |
| タンパク質・核酸の鎖数 | 48 |
| 化学式量合計 | 1370009.17 |
| 構造登録者 | |
| 主引用文献 | Li, F.,Hou, C.D.,Lokareddy, R.K.,Yang, R.,Forti, F.,Briani, F.,Cingolani, G. High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. Nat Commun, 14:4052-4052, 2023 Cited by PubMed Abstract: E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4. PubMed: 37422479DOI: 10.1038/s41467-023-39756-z 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.6 Å) |
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