8ENQ

E. coli CsgA fibril (218-pixel box size)

8ENQ の概要

• エントリーDOI: 10.2210/pdb8enq/pdb
• EMDBエントリー: 28276
• 分子名称: Major curlin subunit (1 entity in total)
• 機能のキーワード: curli, csga fibril, biofilm, protein fibril
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 98670.35

構造登録者

Bu, F.,Liu, B. (登録日: 2022-09-30, 公開日: 2023-10-04, 最終更新日: 2024-04-24)

主引用文献

Bu, F.,Dee, D.R.,Liu, B.
Structural insight into Escherichia coli CsgA amyloid fibril assembly.
Mbio, 15:e0041924-e0041924, 2024

PubMed Abstract: The discovery of functional amyloids in bacteria dates back several decades, and our understanding of the curli biogenesis system has gradually expanded over time. However, due to its high aggregation propensity and intrinsically disordered nature, CsgA, the main structural component of curli fibrils, has eluded comprehensive structural characterization. Recent advancements in cryo-electron microscopy (cryo-EM) offer a promising tool to achieve high-resolution structural insights into CsgA fibrils. In this study, we outline an approach to addressing the colloidal instability challenges associated with CsgA, achieved through engineering and electrostatic repulsion. Then, we present the cryo-EM structure of CsgA fibrils at 3.62 Å resolution. This structure provides new insights into the cross-β structure of CsgA. Additionally, our study identifies two distinct spatial arrangements within several CsgA fibrils, a 2-CsgA-fibril pair and a 3-CsgA-fibril bundle, shedding light on the intricate hierarchy of the biofilm extracellular matrix and laying the foundation for precise manipulation of CsgA-derived biomaterials.IMPORTANCEThe visualization of the architecture of CsgA amyloid fibril has been a longstanding research question, for which a high-resolution structure is still unavailable. CsgA serves as a major subunit of curli, the primary component of the extracellular matrix generated by bacteria. The support provided by this extracellular matrix enables bacterial biofilms to resist antibiotic treatment, significantly impacting human health. CsgA has been identified in members of , with pathogenic being the most well-known model system. Our novel insights into the structure of CsgA protofilaments form the basis for drug design targeting diseases associated with biofilms. Additionally, CsgA is widely researched in biomaterials due to its self-assembly characteristics. The resolved spatial arrangements of CsgA amyloids revealed in our study will further enhance the precision design of functional biomaterials. Therefore, our study uniquely contributes to the understanding of CsgA amyloids for both microbiology and material science.

PubMed: 38501920
DOI: 10.1128/mbio.00419-24

実験手法

ELECTRON MICROSCOPY (3.6 Å)