8EHG

Rabbit muscle aldolase determined using single-particle cryo-EM with Apollo camera.

8EHG の概要

• エントリーDOI: 10.2210/pdb8ehg/pdb
• EMDBエントリー: 28147
• 分子名称: Fructose-bisphosphate aldolase A (1 entity in total)
• 機能のキーワード: glycolysis, sugar binding protein
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 157579.50

構造登録者

Peng, R.,Fu, X.,Mendez, J.H.,Randolph, P.H.,Bammes, B.,Stagg, S.M. (登録日: 2022-09-14, 公開日: 2022-12-21, 最終更新日: 2024-06-19)

主引用文献

Peng, R.,Fu, X.,Mendez, J.H.,Randolph, P.S.,Bammes, B.E.,Stagg, S.M.
Characterizing the resolution and throughput of the Apollo direct electron detector.
J Struct Biol X, 7:100080-100080, 2023

PubMed Abstract: Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been developed by Direct Electron. The Apollo uses a novel event-based MAPS detector custom designed for ultra-fast electron counting. We have evaluated this new camera, finding that it delivers high detective quantum efficiency (DQE) and low coincidence loss, enabling high-quality electron counting data acquisition at up to nearly 80 input electrons per pixel per second. We further characterized the performance of Apollo for single particle cryo-EM on real biological samples. Using mouse apoferritin, Apollo yielded better than 1.9 Å resolution reconstructions at all three tested dose rates from a half-day data collection session each. With longer collection time and improved specimen preparation, mouse apoferritin was reconstructed to 1.66 Å resolution. Applied to a more challenging small protein aldolase, we obtained a 2.24 Å resolution reconstruction. The high quality of the map indicates that the Apollo has sufficiently high DQE to reconstruct smaller proteins and complexes with high-fidelity. Our results demonstrate that the Apollo camera performs well across a broad range of dose rates and is capable of capturing high quality data that produce high-resolution reconstructions for large and small single particle samples.

PubMed: 36578473
DOI: 10.1016/j.yjsbx.2022.100080

実験手法

ELECTRON MICROSCOPY (2.24 Å)