8EEX
Cas7-11 in complex with Csx29
Summary for 8EEX
Entry DOI | 10.2210/pdb8eex/pdb |
EMDB information | 28064 |
Descriptor | Cas7-11, Csx29, crRNA, ... (4 entities in total) |
Functional Keywords | crispr, endonuclease, endopeptidase, rna binding protein-rna complex, rna binding protein/rna |
Biological source | Desulfonema ishimotonii More |
Total number of polymer chains | 3 |
Total formula weight | 283917.26 |
Authors | Demircioglu, F.E.,Wilkinson, M.E.,Strecker, J.,Li, D.,Faure, G.,Macrae, R.K.,Zhang, F. (deposition date: 2022-09-07, release date: 2022-11-16, Last modification date: 2024-06-19) |
Primary citation | Strecker, J.,Demircioglu, F.E.,Li, D.,Faure, G.,Wilkinson, M.E.,Gootenberg, J.S.,Abudayyeh, O.O.,Nishimasu, H.,Macrae, R.K.,Zhang, F. RNA-activated protein cleavage with a CRISPR-associated endopeptidase. Science, 378:874-881, 2022 Cited by PubMed Abstract: In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that there may be other RNA-guided non-nucleolytic enzymes. One such gene from encodes the TPR-CHAT protease Csx29, which is associated with the CRISPR effector Cas7-11. Here, we demonstrate that this CRISPR-associated protease (CASP) exhibits programmable RNA-activated endopeptidase activity against a sigma factor inhibitor to regulate a transcriptional response. Cryo-electron microscopy of an active and substrate-bound CASP complex reveals an allosteric activation mechanism that reorganizes Csx29 catalytic residues upon target RNA binding. This work reveals an RNA-guided function in nature that can be leveraged for RNA-sensing applications in vitro and in human cells. PubMed: 36423276DOI: 10.1126/science.add7450 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.95 Å) |
Structure validation
Download full validation report