8EEA

Structure of E.coli Septu (PtuAB) complex

8EEA の概要

• エントリーDOI: 10.2210/pdb8eea/pdb
• EMDBエントリー: 28048 28049
• 分子名称: PtuA, PtuB, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: ptua, ptub septu, immune system
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 377439.24

構造登録者

Shen, Z.F.,Fu, T.M. (登録日: 2022-09-06, 公開日: 2023-12-27, 最終更新日: 2024-04-03)

主引用文献

Li, Y.,Shen, Z.,Zhang, M.,Yang, X.Y.,Cleary, S.P.,Xie, J.,Marathe, I.A.,Kostelic, M.,Greenwald, J.,Rish, A.D.,Wysocki, V.H.,Chen, C.,Chen, Q.,Fu, T.M.,Yu, Y.
PtuA and PtuB assemble into an inflammasome-like oligomer for anti-phage defense.
Nat.Struct.Mol.Biol., 31:413-423, 2024

PubMed Abstract: Escherichia coli Septu system, an anti-phage defense system, comprises two components: PtuA and PtuB. PtuA contains an ATPase domain, while PtuB is predicted to function as a nuclease. Here we show that PtuA and PtuB form a stable complex with a 6:2 stoichiometry. Cryo-electron microscopy structure of PtuAB reveals a distinctive horseshoe-like configuration. PtuA adopts a hexameric arrangement, organized as an asymmetric trimer of dimers, contrasting the ring-like structure by other ATPases. Notably, the three pairs of PtuA dimers assume distinct conformations and fulfill unique roles in recruiting PtuB. Our functional assays have further illuminated the importance of the oligomeric assembly of PtuAB in anti-phage defense. Moreover, we have uncovered that ATP molecules can directly bind to PtuA and inhibit the activities of PtuAB. Together, the assembly and function of the Septu system shed light on understanding other ATPase-containing systems in bacterial immunity.

PubMed: 38177683
DOI: 10.1038/s41594-023-01172-8

実験手法

ELECTRON MICROSCOPY (2.6 Å)