8EBS

Initial DNA-lesion (Cy5) binding by XPC and TFIIH

8EBS の概要

• エントリーDOI: 10.2210/pdb8ebs/pdb
• 関連するPDBエントリー: 8EBS 8EBT 8EBU 8EBV 8EBW 8EBX 8EBY
• EMDBエントリー: 27996 27997 27998 27999 28000 28001 28002
• 分子名称: TFIIH basal transcription factor complex helicase XPB subunit, Centrin-2, DNA (Cy5), ... (15 entities in total)
• 機能のキーワード: protein-dna complex, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 585993.63

構造登録者

Kim, J.,Yang, W. (登録日: 2022-08-31, 公開日: 2023-04-19, 最終更新日: 2024-06-19)

主引用文献

Kim, J.,Li, C.L.,Chen, X.,Cui, Y.,Golebiowski, F.M.,Wang, H.,Hanaoka, F.,Sugasawa, K.,Yang, W.
Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.
Nature, 617:170-175, 2023

PubMed Abstract: Nucleotide excision repair removes DNA lesions caused by ultraviolet light, cisplatin-like compounds and bulky adducts. After initial recognition by XPC in global genome repair or a stalled RNA polymerase in transcription-coupled repair, damaged DNA is transferred to the seven-subunit TFIIH core complex (Core7) for verification and dual incisions by the XPF and XPG nucleases. Structures capturing lesion recognition by the yeast XPC homologue Rad4 and TFIIH in transcription initiation or DNA repair have been separately reported. How two different lesion recognition pathways converge and how the XPB and XPD helicases of Core7 move the DNA lesion for verification are unclear. Here we report on structures revealing DNA lesion recognition by human XPC and DNA lesion hand-off from XPC to Core7 and XPA. XPA, which binds between XPB and XPD, kinks the DNA duplex and shifts XPC and the DNA lesion by nearly a helical turn relative to Core7. The DNA lesion is thus positioned outside of Core7, as would occur with RNA polymerase. XPB and XPD, which track the lesion-containing strand but translocate DNA in opposite directions, push and pull the lesion-containing strand into XPD for verification.

PubMed: 37076618
DOI: 10.1038/s41586-023-05959-z

実験手法

ELECTRON MICROSCOPY (4 Å)