8EAW

An asymmetric disk assembly formed by tandem dimers of the tobacco mosaic viral capsid protein (TMV)

8EAW の概要

• エントリーDOI: 10.2210/pdb8eaw/pdb
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: viral capsid proteins, tobacco mosaic virus, protein assembly, viral protein
• 由来する生物種: Tobacco mosaic virus (vulgare); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 603438.71

構造登録者

Dai, J.,Pereira, J.H.,Adams, P.D.,Francis, M.B. (登録日: 2022-08-29, 公開日: 2023-03-01, 最終更新日: 2024-05-01)

主引用文献

Dai, J.,Wilhelm, K.B.,Bischoff, A.J.,Pereira, J.H.,Dedeo, M.T.,Garcia-Almedina, D.M.,Adams, P.D.,Groves, J.T.,Francis, M.B.
A Membrane-Associated Light-Harvesting Model is Enabled by Functionalized Assemblies of Gene-Doubled TMV Proteins.
Small, 19:e2207805-e2207805, 2023

PubMed Abstract: Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded within phospholipid membranes. Artificial light-harvesting models are valuable tools for understanding the structural features underpinning energy absorption and transfer within chromophore arrays. Here, a method for attaching a protein-based light-harvesting model to a planar, fluid supported lipid bilayer (SLB) is developed.  The protein model consists of the tobacco mosaic viral capsid proteins that are gene-doubled to create a tandem dimer (dTMV). Assemblies of dTMV break the facial symmetry of the double disk to allow for differentiation between the disk faces. A single reactive lysine residue is incorporated into the dTMV assemblies for the site-selective attachment of chromophores for light absorption. On the opposing dTMV face, a cysteine residue is incorporated for the bioconjugation of a peptide containing a polyhistidine tag for association with SLBs. The dual-modified dTMV complexes show significant association with SLBs and exhibit mobility on the bilayer. The techniques used herein offer a new method for protein-surface attachment and provide a platform for evaluating excited state energy transfer events in a dynamic, fully synthetic artificial light-harvesting system.

PubMed: 36811150
DOI: 10.1002/smll.202207805

実験手法

X-RAY DIFFRACTION (2.8 Å)