8EAM

SsoMCM hexamer bound to Mg/ADP-BeFx and DNA. Class 2. Merged particles from datasets with 3 different DNA entities

これはPDB形式変換不可エントリーです。

8EAM の概要

• エントリーDOI: 10.2210/pdb8eam/pdb
• 関連するPDBエントリー: 8EAF 8EAG 8EAH 8EAI 8EAJ 8EAK 8EAL
• EMDBエントリー: 27974 27975 27976 27977 27978 27979 27980 27981
• 分子名称: Minichromosome maintenance protein MCM, DNA, ZINC ION, ... (6 entities in total)
• 機能のキーワード: helicase, atpase, replication, transferase-dna complex, transferase/dna
• 由来する生物種: Saccharolobus solfataricus P2; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 417915.60

構造登録者

Meagher, M.,Myasnikov, A.,Enemark, E.J. (登録日: 2022-08-29, 公開日: 2022-12-14, 最終更新日: 2024-06-19)

主引用文献

Meagher, M.,Myasnikov, A.,Enemark, E.J.
Two Distinct Modes of DNA Binding by an MCM Helicase Enable DNA Translocation.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: A six-subunit ATPase ring forms the central hub of the replication forks in all domains of life. This ring performs a helicase function to separate the two complementary DNA strands to be replicated and drives the replication machinery along the DNA. Disruption of this helicase/ATPase ring is associated with genetic instability and diseases such as cancer. The helicase/ATPase rings of eukaryotes and archaea consist of six minichromosome maintenance (MCM) proteins. Prior structural studies have shown that MCM rings bind one encircled strand of DNA in a spiral staircase, suggesting that the ring pulls this strand of DNA through its central pore in a hand-over-hand mechanism where the subunit at the bottom of the staircase dissociates from DNA and re-binds DNA one step above the staircase. With high-resolution cryo-EM, we show that the MCM ring of the archaeal organism binds an encircled DNA strand in two different modes with different numbers of subunits engaged to DNA, illustrating a plausible mechanism for the alternating steps of DNA dissociation and re-association that occur during DNA translocation.

PubMed: 36499022
DOI: 10.3390/ijms232314678

実験手法

ELECTRON MICROSCOPY (2.59 Å)