8E5G
Cryo-EM of A. veneficus cytochrome filament
Summary for 8E5G
| Entry DOI | 10.2210/pdb8e5g/pdb |
| EMDB information | 27912 |
| Descriptor | c-type cytochrome, HEME C (2 entities in total) |
| Functional Keywords | helical symmetry, cytochrome filmanet, conductive nanowires, microbial nanowires, electron transport |
| Biological source | Archaeoglobus veneficus |
| Total number of polymer chains | 1 |
| Total formula weight | 30432.68 |
| Authors | Wang, F.,Baquero, D.P.,Krupovic, M.,Egelman, E.H. (deposition date: 2022-08-22, release date: 2023-05-10, Last modification date: 2024-10-23) |
| Primary citation | Baquero, D.P.,Cvirkaite-Krupovic, V.,Hu, S.S.,Fields, J.L.,Liu, X.,Rensing, C.,Egelman, E.H.,Krupovic, M.,Wang, F. Extracellular cytochrome nanowires appear to be ubiquitous in prokaryotes. Cell, 186:2853-2864.e8, 2023 Cited by PubMed Abstract: Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to numerous potential applications. However, whether other organisms employ similar ECNs for electron transfer remains unknown. Here, using cryoelectron microscopy, we describe the atomic structures of two ECNs from two major orders of hyperthermophilic archaea present in deep-sea hydrothermal vents and terrestrial hot springs. Homologs of Archaeoglobus veneficus ECN are widespread among mesophilic methane-oxidizing Methanoperedenaceae, alkane-degrading Syntrophoarchaeales archaea, and in the recently described megaplasmids called Borgs. The ECN protein subunits lack similarities in their folds; however, they share a common heme arrangement, suggesting an evolutionarily optimized heme packing for efficient electron transfer. The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range electron transfer in both prokaryotic domains of life. PubMed: 37290436DOI: 10.1016/j.cell.2023.05.012 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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