8E5G

Cryo-EM of A. veneficus cytochrome filament

8E5G の概要

• エントリーDOI: 10.2210/pdb8e5g/pdb
• EMDBエントリー: 27912
• 分子名称: c-type cytochrome, HEME C (2 entities in total)
• 機能のキーワード: helical symmetry, cytochrome filmanet, conductive nanowires, microbial nanowires, electron transport
• 由来する生物種: Archaeoglobus veneficus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30432.68

構造登録者

Wang, F.,Baquero, D.P.,Krupovic, M.,Egelman, E.H. (登録日: 2022-08-22, 公開日: 2023-05-10, 最終更新日: 2024-10-23)

主引用文献

Baquero, D.P.,Cvirkaite-Krupovic, V.,Hu, S.S.,Fields, J.L.,Liu, X.,Rensing, C.,Egelman, E.H.,Krupovic, M.,Wang, F.
Extracellular cytochrome nanowires appear to be ubiquitous in prokaryotes.
Cell, 186:2853-2864.e8, 2023

PubMed Abstract: Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to numerous potential applications. However, whether other organisms employ similar ECNs for electron transfer remains unknown. Here, using cryoelectron microscopy, we describe the atomic structures of two ECNs from two major orders of hyperthermophilic archaea present in deep-sea hydrothermal vents and terrestrial hot springs. Homologs of Archaeoglobus veneficus ECN are widespread among mesophilic methane-oxidizing Methanoperedenaceae, alkane-degrading Syntrophoarchaeales archaea, and in the recently described megaplasmids called Borgs. The ECN protein subunits lack similarities in their folds; however, they share a common heme arrangement, suggesting an evolutionarily optimized heme packing for efficient electron transfer. The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range electron transfer in both prokaryotic domains of life.

PubMed: 37290436
DOI: 10.1016/j.cell.2023.05.012

実験手法

ELECTRON MICROSCOPY (3.9 Å)