8E1W

Neutron crystal structure of Panus similis AA9A at room temperature

8E1W の概要

• エントリーDOI: 10.2210/pdb8e1w/pdb
• 分子名称: Endo-beta-1,4-glucanase D, COPPER (II) ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: beta sandwiches, glycosylated, oxidoreductase
• 由来する生物種: Panus similis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25593.06

構造登録者

Meilleur, F.,Tandrup, T.,Lo Leggio, L. (登録日: 2022-08-11, 公開日: 2023-01-11, 最終更新日: 2024-04-03)

主引用文献

Tandrup, T.,Lo Leggio, L.,Meilleur, F.
Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature.
Acta Crystallogr.,Sect.F, 79:1-7, 2023

PubMed Abstract: Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO-carbohydrate complexes.

PubMed: 36598350
DOI: 10.1107/S2053230X22011335

実験手法

NEUTRON DIFFRACTION (2.8 Å)
X-RAY DIFFRACTION (2.1 Å)