8DWW
Chikungunya VLP in complex with neutralizing Fab 506.A08 (asymmetric unit)
Summary for 8DWW
| Entry DOI | 10.2210/pdb8dww/pdb |
| EMDB information | 27763 |
| Descriptor | E1 glycoprotein, 506.A08 heavy chain, 506.A08 light chain, ... (6 entities in total) |
| Functional Keywords | antibody, viral protein-immune system complex, csgid, structural genomics, center for structural genomics of infectious diseases, viral protein/immune system |
| Biological source | Chikungunya virus strain Senegal 37997 More |
| Total number of polymer chains | 20 |
| Total formula weight | 543096.95 |
| Authors | Adams, L.J.,Fremont, D.H.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2022-08-02, release date: 2023-05-31) |
| Primary citation | Raju, S.,Adams, L.J.,Earnest, J.T.,Warfield, K.,Vang, L.,Crowe Jr., J.E.,Fremont, D.H.,Diamond, M.S. A chikungunya virus-like particle vaccine induces broadly neutralizing and protective antibodies against alphaviruses in humans. Sci Transl Med, 15:eade8273-eade8273, 2023 Cited by PubMed Abstract: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes epidemics of acute and chronic musculoskeletal disease. Here, we analyzed the human B cell response to a CHIKV-like particle-adjuvanted vaccine (PXVX0317) from samples obtained from a phase 2 clinical trial in humans (NCT03483961). Immunization with PXVX0317 induced high levels of neutralizing antibody in serum against CHIKV and circulating antigen-specific B cells up to 6 months after immunization. Monoclonal antibodies (mAbs) generated from peripheral blood B cells of three PXVX0317-vaccinated individuals on day 57 after immunization potently neutralized CHIKV infection, and a subset of these inhibited multiple related arthritogenic alphaviruses. Epitope mapping and cryo-electron microscopy defined two broadly neutralizing mAbs that uniquely bind to the apex of the B domain of the E2 glycoprotein. These results demonstrate the inhibitory breadth and activity of the human B cell response induced by the PXVX0317 vaccine against CHIKV and potentially other related alphaviruses. PubMed: 37196061DOI: 10.1126/scitranslmed.ade8273 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.13 Å) |
Structure validation
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