8DWW

Chikungunya VLP in complex with neutralizing Fab 506.A08 (asymmetric unit)

8DWW の概要

• エントリーDOI: 10.2210/pdb8dww/pdb
• EMDBエントリー: 27763
• 分子名称: E1 glycoprotein, 506.A08 heavy chain, 506.A08 light chain, ... (6 entities in total)
• 機能のキーワード: antibody, viral protein-immune system complex, csgid, structural genomics, center for structural genomics of infectious diseases, viral protein/immune system
• 由来する生物種: Chikungunya virus strain Senegal 37997; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 543096.95

構造登録者

Adams, L.J.,Fremont, D.H.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2022-08-02, 公開日: 2023-05-31)

主引用文献

Raju, S.,Adams, L.J.,Earnest, J.T.,Warfield, K.,Vang, L.,Crowe Jr., J.E.,Fremont, D.H.,Diamond, M.S.
A chikungunya virus-like particle vaccine induces broadly neutralizing and protective antibodies against alphaviruses in humans.
Sci Transl Med, 15:eade8273-eade8273, 2023

PubMed Abstract: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes epidemics of acute and chronic musculoskeletal disease. Here, we analyzed the human B cell response to a CHIKV-like particle-adjuvanted vaccine (PXVX0317) from samples obtained from a phase 2 clinical trial in humans (NCT03483961). Immunization with PXVX0317 induced high levels of neutralizing antibody in serum against CHIKV and circulating antigen-specific B cells up to 6 months after immunization. Monoclonal antibodies (mAbs) generated from peripheral blood B cells of three PXVX0317-vaccinated individuals on day 57 after immunization potently neutralized CHIKV infection, and a subset of these inhibited multiple related arthritogenic alphaviruses. Epitope mapping and cryo-electron microscopy defined two broadly neutralizing mAbs that uniquely bind to the apex of the B domain of the E2 glycoprotein. These results demonstrate the inhibitory breadth and activity of the human B cell response induced by the PXVX0317 vaccine against CHIKV and potentially other related alphaviruses.

PubMed: 37196061
DOI: 10.1126/scitranslmed.ade8273

実験手法

ELECTRON MICROSCOPY (3.13 Å)