8DW4

Glycosylase MutY variant N146S in complex with DNA containing d(8-oxo-G) paired with an abasic site product (AP) generated by the enzyme in crystals by removal of calcium

Summary for 8DW4

• Entry DOI: 10.2210/pdb8dw4/pdb
• Related: 8DVP
• Descriptor: Adenine DNA glycosylase, DNA (5'-D(*AP*AP*GP*AP*CP*(8OG)P*TP*GP*GP*AP*C)-3'), DNA (5'-D(P*GP*TP*CP*CP*AP*(AAB)P*GP*TP*CP*T)-3'), ... (7 entities in total)
• Functional Keywords: protein-dna complex, dna repair, apurinic/apyrimidinic, base excision repair, hydrolase, hydrolase-dna complex, hydrolase/dna
• Biological source: Geobacillus stearothermophilus; More
• Total number of polymer chains: 3
• Total formula weight: 48810.57

Authors

Demir, M.,Russelburg, L.P.,Horvath, M.P.,David, S.S. (deposition date: 2022-07-31, release date: 2022-11-30, Last modification date: 2023-10-25)

• Primary citation: Demir, M.,Russelburg, L.P.,Lin, W.J.,Trasvina-Arenas, C.H.,Huang, B.,Yuen, P.K.,Horvath, M.P.,David, S.S.; Structural snapshots of base excision by the cancer-associated variant MutY N146S reveal a retaining mechanism.; Nucleic Acids Res., 51:1034-1049, 2023; PubMed: 36631987; DOI: 10.1093/nar/gkac1246

Experimental method

X-RAY DIFFRACTION (2.49 Å)