8DU7

Room-temperature serial synchrotron crystallography (SSX) structure of apo PTP1B

8DU7 の概要

• エントリーDOI: 10.2210/pdb8du7/pdb
• 分子名称: Tyrosine-protein phosphatase non-receptor type 1, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: protein tyrosine phosphatase, ptp, protein tyrosine phosphatase 1b, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33150.92

構造登録者

Ebrahim, A.,Sharma, S.,Keedy, D.A. (登録日: 2022-07-27, 公開日: 2022-08-17, 最終更新日: 2023-10-25)

主引用文献

Sharma, S.,Ebrahim, A.,Keedy, D.A.
Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B.
Acta Crystallogr.,Sect.F, 79:23-30, 2023

PubMed Abstract: Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. Here, a recently introduced serial sample-support chip system has been used to obtain the first SSX structure of a human phosphatase, specifically protein tyrosine phosphatase 1B (PTP1B) in the unliganded (apo) state. In previous apo room-temperature structures, the active site and allosteric sites adopted alternate conformations, including open and closed conformations of the active-site WPD loop and of a distal allosteric site. By contrast, in our SSX structure the active site is best fitted with a single conformation, but the distal allosteric site is best fitted with alternate conformations. This observation argues for additional nuance in interpreting the nature of allosteric coupling in this protein. Overall, our results illustrate the promise of serial methods for room-temperature crystallography, as well as future avant-garde crystallography experiments, for PTP1B and other proteins.

PubMed: 36598353
DOI: 10.1107/S2053230X22011645

実験手法

X-RAY DIFFRACTION (2.4 Å)