8DSD

Human NAMPT in complex with substrate NAM and small molecule activator NP-A1-S

8DSD の概要

• エントリーDOI: 10.2210/pdb8dsd/pdb
• 分子名称: Nicotinamide phosphoribosyltransferase, NICOTINAMIDE, PHOSPHATE ION, ... (7 entities in total)
• 機能のキーワード: nad biosynthesis enzyme:activator complex, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 114910.01

構造登録者

Ratia, K.,Xiong, R.,Shen, Z.,Thatcher, G.R. (登録日: 2022-07-22, 公開日: 2023-03-08, 最終更新日: 2023-10-25)

主引用文献

Ratia, K.M.,Shen, Z.,Gordon-Blake, J.,Lee, H.,Laham, M.S.,Krider, I.S.,Christie, N.,Ackerman-Berrier, M.,Penton, C.,Knowles, N.G.,Musku, S.R.,Fu, J.,Velma, G.R.,Xiong, R.,Thatcher, G.R.J.
Mechanism of Allosteric Modulation of Nicotinamide Phosphoribosyltransferase to Elevate Cellular NAD.
Biochemistry, 62:923-933, 2023

PubMed Abstract: In aging and disease, cellular nicotinamide adenine dinucleotide (NAD) is depleted by catabolism to nicotinamide (NAM). NAD supplementation is being pursued to enhance human healthspan and lifespan. Activation of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting step in NAD biosynthesis, has the potential to increase the salvage of NAM. Novel NAMPT-positive allosteric modulators (N-PAMs) were discovered in addition to the demonstration of NAMPT activation by biogenic phenols. The mechanism of activation was revealed through the synthesis of novel chemical probes, new NAMPT co-crystal structures, and enzyme kinetics. Binding to a rear channel in NAMPT regulates NAM binding and turnover, with biochemical observations being replicated by NAD measurements in human cells. The mechanism of action of N-PAMs identifies, for the first time, the role of the rear channel in the regulation of NAMPT turnover coupled to productive and nonproductive NAM binding. The tight regulation of cellular NAMPT via feedback inhibition by NAM, NAD, and adenosine 5'-triphosphate (ATP) is differentially regulated by N-PAMs and other activators, indicating that different classes of pharmacological activators may be engineered to restore or enhance NAD levels in affected tissues.

PubMed: 36746631
DOI: 10.1021/acs.biochem.2c00655

実験手法

X-RAY DIFFRACTION (1.429 Å)