8DSB

Lambda Bacteriophage Orf63

8DSB の概要

• エントリーDOI: 10.2210/pdb8dsb/pdb
• NMR情報: BMRB: 51519
• 分子名称: Xis (Excision72) (1 entity in total)
• 機能のキーワード: bacteriophage, lytic development, viral protein
• 由来する生物種: Lambdavirus lambda (Bacteriophage lambda)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17715.78

構造登録者

Donaldson, L.W. (登録日: 2022-07-22, 公開日: 2023-07-26, 最終更新日: 2024-05-01)

主引用文献

Dydecka, A.,Bloch, S.,Rizvi, A.,Perez, S.,Nejman-Falenczyk, B.,Topka, G.,Gasior, T.,Necel, A.,Wegrzyn, G.,Donaldson, L.W.,Wegrzyn, A.
Bad Phages in Good Bacteria: Role of the Mysterious orf63 of lambda and Shiga Toxin-Converting Phi 24 B Bacteriophages.
Front Microbiol, 8:1618-1618, 2017

PubMed Abstract: Lambdoid bacteriophages form a group of viruses that shares a common schema of genome organization and lifecycle. Some of them can play crucial roles in creating the pathogenic profiles of strains. For example, Shiga toxin-producing (STEC) acquired genes, encoding Shiga toxins, via lambdoid prophages (Stx phages). The results obtained so far present the evidence for the relation between the region of the phage genome and lambdoid phage development, however molecular mechanisms of activities of the genes' products are still unknown. In view of this, we decided to determine the influence of the uncharacterized open reading frame of the region on lambdoid phages development using recombinant prophages, λ and Stx phage Φ24 We have demonstrated that codes for a folded protein, thus, it is a functional gene. NMR spectroscopy and analytical gel filtration were used to extend this observation further. From backbone chemical shifts, Orf63 is oligomeric in solution, likely a trimer and consistent with its small size (63 aa.), is comprised of two helices, likely intertwined to form the oligomer. We observed that the deletion of phage does not impair the intracellular lambdoid phage lytic development, however delays the time and decreases the efficiency of prophage induction and in consequence results in increased survival of during phage lytic development. Additionally, the deletion of phage negatively influences expression of the major phage genes and open reading frames from the region during prophage induction with hydrogen peroxide. We conclude, that lambdoid phage may have specific functions in the regulation of lambdoid phages development, especially at the stage of the lysis vs. lysogenization decision. Besides, probably participates in the regulation of the level of expression of essential phage genes and open reading frames from the region during prophage induction.

PubMed: 28890713
DOI: 10.3389/fmicb.2017.01618

実験手法

SOLUTION NMR