8DQU

Nanobody bound SARS-CoV-2 Nsp9

8DQU の概要

• エントリーDOI: 10.2210/pdb8dqu/pdb
• 分子名称: Nanobody, Non-structural protein 9 (3 entities in total)
• 機能のキーワード: sars-cov-2, nsp9, nanobody, viral protein
• 由来する生物種: Lama glama; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 56691.53

構造登録者

Littler, D.R.,Gully, B.S.,Rossjohn, J. (登録日: 2022-07-20, 公開日: 2023-02-08, 最終更新日: 2024-11-06)

主引用文献

Pan, Y.,Chandrashekaran, I.R.,Tennant, L.,Rossjohn, J.,Littler, D.R.
Inside-out: Antibody-binding reveals potential folding hinge-points within the SARS-CoV-2 replication co-factor nsp9.
Plos One, 18:e0283194-e0283194, 2023

PubMed Abstract: Nsp9 is a conserved accessory component of the coronaviral replication and transcription complex. It is the predominant substrate of nsp12's nucleotidylation activity while also serving to recruit proteins required for viral 5'-capping. Anti-nsp9 specific nanobodies have been isolated previously. We confirm that their binding mode is centred upon Trp-53 within SARS-CoV-2 nsp9. Antibody binding at this site surprisingly results in large-scale changes to the overall topology of this coronaviral unique fold. We further characterise the antibody-induced structural dynamism within nsp9, identifying a number of potentially flexible regions. A large expansion of the cavity between the s2-s3 and s4-s5 loops is particularly noteworthy. As is the potential for large-scale movements in the C-terminal GxxxG helix.

PubMed: 37036856
DOI: 10.1371/journal.pone.0283194

実験手法

X-RAY DIFFRACTION (2.45003185971 Å)