8DOI

Dehaloperoxidase B in complex with 2,2'-Biphenol

Summary for 8DOI

• Entry DOI: 10.2210/pdb8doi/pdb
• Descriptor: Dehaloperoxidase B, PROTOPORPHYRIN IX CONTAINING FE, 2-(2-hydroxyphenyl)phenol, ... (8 entities in total)
• Functional Keywords: dehaloperoxidase b, heme peroxidase, peroxigenase, oxygen binding, heme cofactor oxidorductase, oxidoreductase
• Biological source: Amphitrite ornata
• Total number of polymer chains: 2
• Total formula weight: 33809.76

Authors

de Serrano, V.S.,Yun, D.,Ghiladi, R. (deposition date: 2022-07-13, release date: 2022-11-02, Last modification date: 2023-10-25)

Primary citation

Yun, D.,de Serrano, V.,Ghiladi, R.A.
Oxidation of bisphenol A (BPA) and related compounds by the multifunctional catalytic globin dehaloperoxidase.
J.Inorg.Biochem., 238:112020-112020, 2023

PubMed Abstract: Dehaloperoxidase (DHP) from the marine polychaete Amphitrite ornata is a multifunctional enzyme that possesses peroxidase, peroxygenase, oxidase and oxygenase activities. Herein, we investigated the reactivity of DHP B with bisphenol A (BPA) and related compounds (bisphenol E, bisphenol F, tetrachlorobisphenol A, 2,2'-biphenol, 3,3'-biphenol, 4,4'-biphenol, and 3,3'-dibromo-4,4'-biphenol). As a previously unknown substrate for DHP B, BPA (as a representative substrate) is an endocrine disruptor widely used in polycarbonate and epoxy resins, thus resulting in human exposure. Reactivity studies with these substrates were investigated using high performance liquid chromatography (HPLC), and their corresponding oxidation products were determined by mass spectrometry (GC-MS/ LC-MS). BPA undergoes oxidation in the presence of DHP B and hydrogen peroxide yielding two cleavage products (4-isopropenylphenol and 4-(2-hydroxypropan-2-yl)phenol), and oligomers with varying degrees of oxidation. O-labeling studies confirmed that the O-atom incorporated into the products was derived exclusively from water, consistent with substrate oxidation via a peroxidase-based mechanism. The X-ray crystal structures of DHP bound with bisphenol E (1.48 Å), bisphenol F (1.75 Å), 2,2'-biphenol (1.90 Å) and 3,3'-biphenol (1.30 Å) showed substrate binding sites are in the distal pocket of the heme cofactor, similar to other previously studied DHP substrates. Stopped-flow UV-visible spectroscopy was utilized to investigate the mechanistic details and enzyme oxidation states during substrate turnover, and a reaction mechanism is proposed. The data presented here strongly suggest that DHP B can catalyze the oxidation of bisphenols and biphenols, thus providing evidence of how infaunal invertebrates can contribute to the biotransformation of these marine pollutants.

PubMed: 36272837
DOI: 10.1016/j.jinorgbio.2022.112020

Experimental method

X-RAY DIFFRACTION (1.93 Å)