8DNM

Human Brain Dihydropyrimidinase-related protein 2

8DNM の概要

• エントリーDOI: 10.2210/pdb8dnm/pdb
• EMDBエントリー: 27574
• 分子名称: Dihydropyrimidinase-related protein 2 (1 entity in total)
• 機能のキーワード: human brain, dpysl2, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 249461.66

構造登録者

Tringides, M.L. (登録日: 2022-07-11, 公開日: 2022-11-16, 最終更新日: 2025-06-04)

主引用文献

Tringides, M.L.,Zhang, Z.,Morgan, C.E.,Su, C.C.,Yu, E.W.
A cryo-electron microscopic approach to elucidate protein structures from human brain microsomes.
Life Sci Alliance, 6:-, 2023

PubMed Abstract: We recently developed a "Build and Retrieve" cryo-electron microscopy (cryo-EM) methodology, which is capable of simultaneously producing near-atomic resolution cryo-EM maps for several individual proteins from a heterogeneous, multiprotein sample. Here we report the use of "Build and Retrieve" to define the composition of a raw human brain microsomal lysate. From this sample, we simultaneously identify and solve cryo-EM structures of five different brain enzymes whose functions affect neurotransmitter recycling, iron metabolism, glycolysis, axonal development, energy homeostasis, and retinoic acid biosynthesis. Interestingly, malfunction of these important proteins has been directly linked to several neurodegenerative disorders, such as Alzheimer's, Huntington's, and Parkinson's diseases. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level.

PubMed: 36450447
DOI: 10.26508/lsa.202201724

実験手法

ELECTRON MICROSCOPY (2.76 Å)