8DN9

Acidipropionibacterium acidipropionici encapsulin in a closed state at pH 7.5

8DN9 の概要

• エントリーDOI: 10.2210/pdb8dn9/pdb
• EMDBエントリー: 27558
• 分子名称: 29 kDa antigen cfp29 (1 entity in total)
• 機能のキーワード: encapsulin, virus like particle
• 由来する生物種: Acidipropionibacterium acidipropionici ATCC 4875
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28522.72

構造登録者

Jones, J.A.,Andreas, M.P.,Giessen, T.W. (登録日: 2022-07-11, 公開日: 2023-03-22, 最終更新日: 2024-06-12)

主引用文献

Jones, J.A.,Andreas, M.P.,Giessen, T.W.
Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage.
Biomacromolecules, 24:1388-1399, 2023

PubMed Abstract: Encapsulins are microbial protein nanocages capable of efficient self-assembly and cargo enzyme encapsulation. Due to their favorable properties, including high thermostability, protease resistance, and robust heterologous expression, encapsulins have become popular bioengineering tools for applications in medicine, catalysis, and nanotechnology. Resistance against physicochemical extremes like high temperature and low pH is a highly desirable feature for many biotechnological applications. However, no systematic search for acid-stable encapsulins has been carried out, while the influence of pH on encapsulin shells has so far not been thoroughly explored. Here, we report on a newly identified encapsulin nanocage from the acid-tolerant bacterium . Using transmission electron microscopy, dynamic light scattering, and proteolytic assays, we demonstrate its extreme acid tolerance and resilience against proteases. We structurally characterize the novel nanocage using cryo-electron microscopy, revealing a dynamic five-fold pore that displays distinct "closed" and "open" states at neutral pH but only a singular "closed" state under strongly acidic conditions. Further, the "open" state exhibits the largest pore in an encapsulin shell reported to date. Non-native protein encapsulation capabilities are demonstrated, and the influence of external pH on internalized cargo is explored. Our results expand the biotechnological application range of encapsulin nanocages toward potential uses under strongly acidic conditions and highlight pH-responsive encapsulin pore dynamics.

PubMed: 36796007
DOI: 10.1021/acs.biomac.2c01424

実験手法

ELECTRON MICROSCOPY (2.9 Å)